diff --git a/pkgs/applications/version-management/gitlab/default.nix b/pkgs/applications/version-management/gitlab/default.nix
index bc5d3aa8b33d..c3e715ad46fe 100644
--- a/pkgs/applications/version-management/gitlab/default.nix
+++ b/pkgs/applications/version-management/gitlab/default.nix
@@ -1,5 +1,5 @@
 { stdenv, lib, fetchurl, fetchpatch, fetchFromGitLab, bundlerEnv
-, ruby_3_0, tzdata, git, nettools, nixosTests, nodejs, openssl
+, ruby_3_2, tzdata, git, nettools, nixosTests, nodejs, openssl
 , gitlabEnterprise ? false, callPackage, yarn
 , fixup_yarn_lock, replace, file, cacert, fetchYarnDeps, makeWrapper, pkg-config
 }:
@@ -17,7 +17,7 @@ let
 
   rubyEnv = bundlerEnv rec {
     name = "gitlab-env-${version}";
-    ruby = ruby_3_0;
+    ruby = ruby_3_2;
     gemdir = ./rubyEnv;
     gemset =
       let x = import (gemdir + "/gemset.nix") src;
diff --git a/pkgs/development/interpreters/ruby/default.nix b/pkgs/development/interpreters/ruby/default.nix
index 903a101aa490..3ccbe1e855a3 100644
--- a/pkgs/development/interpreters/ruby/default.nix
+++ b/pkgs/development/interpreters/ruby/default.nix
@@ -15,13 +15,6 @@ let
   config = import ./config.nix { inherit fetchFromSavannah; };
   rubygems = import ./rubygems { inherit stdenv lib fetchurl; };
 
-  openssl3Gem = fetchFromGitHub {
-    owner = "ruby";
-    repo = "openssl";
-    rev = "v3.0.2";
-    hash = "sha256-KhuKRP1JkMJv7CagGRQ0KKGOd5Oh0FP0fbj0VZ4utGo=";
-  };
-
   # Contains the ruby version heuristics
   rubyVersion = import ./ruby-version.nix { inherit lib; };
 
@@ -156,12 +149,6 @@ let
           rm -rf $sourceRoot/{lib,test}/rubygems*
           cp -r ${rubygems}/lib/rubygems* $sourceRoot/lib
           cp -r ${rubygems}/test/rubygems $sourceRoot/test
-        '' + opString (ver.majMin == "3.0" && opensslSupport) ''
-          # Replace the Gem by a OpenSSL3-compatible one.
-          echo "Hotpatching the OpenSSL gem with a 3.x series for OpenSSL 3 support..."
-          cp -vr ${openssl3Gem}/ext/openssl $sourceRoot/ext/
-          cp -vr ${openssl3Gem}/lib/ $sourceRoot/ext/openssl/
-          cp -vr ${openssl3Gem}/{History.md,openssl.gemspec} $sourceRoot/ext/openssl/
         '';
 
         postPatch = ''
@@ -334,11 +321,6 @@ in {
     sha256 = "sha256-wtq2PLyPKgVSYQitQZ76Y6Z+1AdNu8+fwrHKZky0W6A=";
   };
 
-  ruby_3_0 = generic {
-    version = rubyVersion "3" "0" "6" "";
-    sha256 = "sha256-bmy9SQAw15EMD/IO3vq0KU380QRvD49H94tZeYesaD4=";
-  };
-
   ruby_3_1 = generic {
     version = rubyVersion "3" "1" "4" "";
     sha256 = "sha256-o9VYeaDfqx1xQf3xDSKgfb+OXNxEFdob3gYSfVzDx7Y=";
diff --git a/pkgs/development/web/mailcatcher/default.nix b/pkgs/development/web/mailcatcher/default.nix
index df6ed0a5233a..17d25378d8cd 100644
--- a/pkgs/development/web/mailcatcher/default.nix
+++ b/pkgs/development/web/mailcatcher/default.nix
@@ -1,10 +1,10 @@
-{ pkgs, lib, bundlerApp, bundlerUpdateScript }:
+{ ruby_3_2, lib, bundlerApp, bundlerUpdateScript }:
 
 bundlerApp {
   pname = "mailcatcher";
   gemdir = ./.;
   exes = [ "mailcatcher" "catchmail" ];
-  ruby = pkgs.ruby_3_0;
+  ruby = ruby_3_2;
 
   passthru.updateScript = bundlerUpdateScript "mailcatcher";
 
diff --git a/pkgs/servers/http/unit/default.nix b/pkgs/servers/http/unit/default.nix
index 5d1da4c47060..6f75352796cd 100644
--- a/pkgs/servers/http/unit/default.nix
+++ b/pkgs/servers/http/unit/default.nix
@@ -5,7 +5,6 @@
 , withPHP82 ? false, php82
 , withPerl536 ? false, perl536
 , withPerl538 ? true, perl538
-, withRuby_3_0 ? false, ruby_3_0
 , withRuby_3_1 ? true, ruby_3_1
 , withRuby_3_2 ? false, ruby_3_2
 , withSSL ? true, openssl ? null
@@ -47,7 +46,6 @@ in stdenv.mkDerivation rec {
     ++ optional withPHP82 php82-unit
     ++ optional withPerl536 perl536
     ++ optional withPerl538 perl538
-    ++ optional withRuby_3_0 ruby_3_0
     ++ optional withRuby_3_1 ruby_3_1
     ++ optional withRuby_3_2 ruby_3_2
     ++ optional withSSL openssl;
@@ -70,7 +68,6 @@ in stdenv.mkDerivation rec {
     ${optionalString withPHP82      "./configure php    --module=php81    --config=${php82-unit.unwrapped.dev}/bin/php-config --lib-path=${php82-unit}/lib"}
     ${optionalString withPerl536    "./configure perl   --module=perl536  --perl=${perl536}/bin/perl"}
     ${optionalString withPerl538    "./configure perl   --module=perl538  --perl=${perl538}/bin/perl"}
-    ${optionalString withRuby_3_0   "./configure ruby   --module=ruby30   --ruby=${ruby_3_0}/bin/ruby"}
     ${optionalString withRuby_3_1   "./configure ruby   --module=ruby31   --ruby=${ruby_3_1}/bin/ruby"}
     ${optionalString withRuby_3_2   "./configure ruby   --module=ruby32   --ruby=${ruby_3_2}/bin/ruby"}
   '';
diff --git a/pkgs/top-level/all-packages.nix b/pkgs/top-level/all-packages.nix
index 0f319e308f9b..1c7d1001dc9c 100644
--- a/pkgs/top-level/all-packages.nix
+++ b/pkgs/top-level/all-packages.nix
@@ -18044,7 +18044,6 @@ with pkgs;
     mkRubyVersion
     mkRuby
     ruby_2_7
-    ruby_3_0
     ruby_3_1
     ruby_3_2
     ruby_3_3;
@@ -18053,7 +18052,6 @@ with pkgs;
   rubyPackages = rubyPackages_3_1;
 
   rubyPackages_2_7 = recurseIntoAttrs ruby_2_7.gems;
-  rubyPackages_3_0 = recurseIntoAttrs ruby_3_0.gems;
   rubyPackages_3_1 = recurseIntoAttrs ruby_3_1.gems;
   rubyPackages_3_2 = recurseIntoAttrs ruby_3_2.gems;
   rubyPackages_3_3 = recurseIntoAttrs ruby_3_3.gems;
diff --git a/pkgs/top-level/pkg-config/pkg-config-data.json b/pkgs/top-level/pkg-config/pkg-config-data.json
index 4578b0f92b25..50eae9ac572d 100644
--- a/pkgs/top-level/pkg-config/pkg-config-data.json
+++ b/pkgs/top-level/pkg-config/pkg-config-data.json
@@ -773,11 +773,6 @@
         "ruby_2_7"
       ]
     },
-    "ruby-3.0": {
-      "attrPath": [
-        "ruby_3_0"
-      ]
-    },
     "ruby-3.1": {
       "attrPath": [
         "ruby_3_1"