diff --git a/pkgs/development/python-modules/ariadne/default.nix b/pkgs/development/python-modules/ariadne/default.nix
index 3810934eefeb..0e0bf1140a64 100644
--- a/pkgs/development/python-modules/ariadne/default.nix
+++ b/pkgs/development/python-modules/ariadne/default.nix
@@ -4,7 +4,6 @@
 , hatchling
 , freezegun
 , graphql-core
-, opentracing
 , pytest-asyncio
 , pytest-mock
 , pytestCheckHook
@@ -28,6 +27,9 @@ buildPythonPackage rec {
     rev = "refs/tags/${version}";
     hash = "sha256-v3CaLMTo/zbNEoE3K+aWnFTCgLetcnN7vOU/sFqLq2k=";
   };
+  patches = [
+    ./remove-opentracing.patch
+  ];
 
   nativeBuildInputs = [
     hatchling
@@ -41,7 +43,6 @@ buildPythonPackage rec {
 
   nativeCheckInputs = [
     freezegun
-    opentracing
     pytest-asyncio
     pytest-mock
     pytestCheckHook
@@ -58,12 +59,18 @@ buildPythonPackage rec {
     "test_attempt_parse_request_missing_content_type_raises_bad_request_error"
     "test_attempt_parse_non_json_request_raises_bad_request_error"
     "test_attempt_parse_non_json_request_body_raises_bad_request_error"
+    # opentracing
+    "test_query_is_executed_for_multipart_form_request_with_file"
+    "test_query_is_executed_for_multipart_request_with_large_file_with_tracing"
   ];
 
   disabledTestPaths = [
     # missing graphql-sync-dataloader test dep
     "tests/test_dataloaders.py"
     "tests/wsgi/test_configuration.py"
+    # both include opentracing module, which has been removed from nixpkgs
+    "tests/tracing/test_opentracing.py"
+    "tests/tracing/test_opentelemetry.py"
   ];
 
   meta = with lib; {
diff --git a/pkgs/development/python-modules/ariadne/remove-opentracing.patch b/pkgs/development/python-modules/ariadne/remove-opentracing.patch
new file mode 100644
index 000000000000..eecbfafea9b9
--- /dev/null
+++ b/pkgs/development/python-modules/ariadne/remove-opentracing.patch
@@ -0,0 +1,12 @@
+diff --git a/tests/asgi/conftest.py b/tests/asgi/conftest.py
+index a703466..223586e 100644
+--- a/tests/asgi/conftest.py
++++ b/tests/asgi/conftest.py
+@@ -8,7 +8,6 @@ from ariadne.asgi.handlers import (
+     GraphQLTransportWSHandler,
+     GraphQLWSHandler,
+ )
+-from ariadne.contrib.tracing.opentracing import opentracing_extension
+ 
+ 
+ @pytest.fixture